This study explored the personality trait, perception of organizational innovative climate, and innovative behavior for the R&D employees of the communication and networking industry in Taiwan. Specifically, the relationships between personality traits and innovative behavior, and the mediating effects of organizational innovative climate are tested. Data from 206 R&D employees from public-traded companies of communication and the Internet industry are collected before a series of hierarchical regression analyses are conducted to test the hypotheses. The results showed that the personality traits of the R&D employees had correlation with the innovative behavior and organizational innovative climate. In addition, the organizational innovative climate of the R&D employees had a significant and positive correlation with innovative behavior. Finally, the perception of organizational innovative climate of the R&D employees had partial mediation effect in the relationship between personality traits and innovative behavior.