Estradiol upregulates calcineurin expression via overexpression of estrogen receptor alpha gene in systemic lupus erythematosus

MUIR > Graduate Institute of Health Care > Department of Food Science and Nutrition > Papers > Item 987654321/1103

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Title:	Estradiol upregulates calcineurin expression via overexpression of estrogen receptor alpha gene in systemic lupus erythematosus 全身性紅斑狼瘡之二羥雌激素調升蛋白質磷酸酶3 CA之表現是經由雌激素受體a基因之過度表現
Authors:	Hui-Li Lin;Jeng-Hsien Yen;Shi-Shin Chiou;Wen-Chan Tsai;Tsan-Teng Ou;Cheng-Chin Wu;Hong-Wen Liu 林慧麗;顏正賢;邱世欣;蔡文展;歐燦騰;吳正欽;劉宏文
Contributors:	Department of Food and Nutrition, Meiho University, Pingtung, Taiwan;Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
Keywords:	蛋白質磷酸酶;雌激素;雌激素受體a基因;全身性紅斑狼瘡
Date:	2011-02
Issue Date:	2011-09-16T05:22:22Z (UTC)
Abstract:	全身性紅斑狼瘡是一種自體免疫疾病，主要侵犯女性為主(男女比為1:9)。為探討女性雌激素如何影响紅斑狼瘡偏向女性發生而研究狼瘡病人與正常人週邊血液淋巴球之雌激素受體a 基因與蛋白的表現。我們收集了20 位女性之紅斑狼瘡病人與6位正常女性志願者為研究對象。雌激素受體a蛋白的表現是以流式細胞測定儀定量之。雌激素受體a 和蛋白質磷酸酶3CA 信息核醣核酸之測定是以半定量之逆轉錄鏈聚合反應測定之。蛋白質磷酸酶3CA 之活度是以蛋白質磷酸酶分析用成套工具測定之。結果發現紅斑狼瘡周邊血淋巴球雌激素受體a之信息核醣核酸及蛋白的表現比正常人有意義增加，其p 值分別為0.001和0.023。此外基礎之蛋白質磷酸酶3CA 表現在紅斑狼瘡淋巴球比正常人有意義的增加，p 值Z0.000。雌激素誘發之蛋白質磷酸酶3CA 表現在紅斑狼瘡淋巴球比正常人有意義的增加(pZ0.007), 其增加為3.15 倍, 此增加可被雌激素受體拮抗者ICI 182,780 抑制。雌激素受體拮抗現象亦發生於蛋白質磷酸酶3CA 之磷酸酶活度。由以上結果顯示雌激素受體a 基因的過度表現以及淋巴球因雌激素之刺激所導致蛋白質磷酸酶3CA 之高度活化結果對狼瘡病人之偏向女性之致病機轉有所貢獻。
Relation:	Kaohsiung Journal of Medical Sciences (2011) 27, 125e131
Appears in Collections:	[Department of Food Science and Nutrition] Papers

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