Autologous bone marrow cell implantation attenuates left ventricular remodeling and improves heart function in porcine myocardial infarction: An echocardiographic, six-month angiographic, and molecular–cellular study

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Title:	Autologous bone marrow cell implantation attenuates left ventricular remodeling and improves heart function in porcine myocardial infarction: An echocardiographic, six-month angiographic, and molecular–cellular study
Authors:	Steve Leu;Cheuk-Kwan Sun;Jiunn-Jye Sheu;Li-Teh Chang;Chun-Man Yuen;Chia-Hung Yen;Chiang-Hua Chiang;Sheung-Fat Ko;Sung-Nan Pei;Sarah Chua;Ali A. Youssef;Chiung-Jen Wu;Hon-Kan Yip
Contributors:	Department of Cardiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of General Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of Cardiovascular Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Basic Science, Nursing Department, Meiho Institute of Technology, Pingtung, Taiwan, ROC;Department of Neurosurgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC f Department of Life Science, National Pingtung University of Science and Technology, Taiwan, ROC;Department of Veterinary Medicine, National Pingtung University of Science and Technology, Taiwan, ROC;Department of Radiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of Hematology;Center for Translational Research in Biomedical Sciences, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of Cardiology, Suez Canal University Hospital, Ismailia, Egypt
Keywords:	Acute myocardial infarct;Autologous bone marrow-derived stem cell therapy;Molecular–cellular mechanisms;Mini-pigs
Date:	2011
Issue Date:	2011-09-27T02:46:11Z (UTC)
Abstract:	Background: We investigated the potential beneﬁts and the underlying mechanisms of autologous bone marrow-derived mononuclear cell (BMDMNC) implantation in a porcine model of acute anterior wall myocardial infarction (AAWMI) by studying 6-month left ventricular (LV) function and LV remodeling. Methods: After being aspirated from the iliac crest and cultured for 1 week, BMDMNCs were implanted immediately after AAWMI induction through the left anterior descending artery ligation. Thirty male minipigs (16–18 kg) were equally divided into group 1 [AAWMI plus saline injection into infarct-ischemia area (IA)], group 2 (AAWMI plus 3.0×107 BMDMNC transplantation into non-IA), group 3 (AAWMI plus 3.0×107 BMDMNC transplantation into IA), group 4 (sham control plus 3.0×107 BMDMNC transplantation into LV myocardium), and group 5 (normal control). Results: By day 90, echocardiography demonstrated an increased LV end-diastolic and end-systolic dimensions but reduced LV ejection fraction (LVEF) in groups 1 and 2 than in other groups (all pb0.01). Six-month angiographic study showed a lower LVEF and wall motion score but a higher mitral regurgitation in groups 1 and 2 than in other groups (all pb0.01). In IA and peri-infarct area, the number of small vessels and mRNA expressions of endothelial nitric oxide synthase, Bcl-2, interleukin (IL)-10, and peroxisome proliferatoractivated receptor-γ coactivator-1α were lower, whereas the number of apoptotic nuclei, caspase-3, Bax, endothelin-1, IL-8, and matrix metalloproteinase was higher in groups 1 and 2 than in other groups (all pb0.01). Conclusions: Autologous BMDMNC transplantation into IA rather non-IA improves LV function and reduces LV remodeling via eliciting a broad-spectrum of molecular–cellular defensive mechanisms.
Relation:	International Journal of Cardiology 150 (2011) 156–168
Appears in Collections:	[Department of Nursing] Papers

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