Meiho University Institutional Repository:Item 987654321/1366
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    题名: The antifungal mechanism of Bacillus subtilis against Pestalotiopsis eugeniae infected to wax apple and development for commercial application.
    作者: Hsiu Fen Lin;Tsang Hai Chen;Shan Da Liu
    日期: 2011-07
    上传时间: 2011-10-19T09:01:50Z (UTC)
    摘要: The inhibition zones of three Bacillus subtilis isolates, BS-99, BS-23857 and BS-33608 against
    Pestalotiopsis eugeniae were, 13.5, 0, and 0 mm, respectively. BS-99 showed the strongest inhibitoryactivity, whereas, no inhibitory activity was reported for BS-23857 or BS-33608. The spore germinationrates of P. eugeniae were not significantly inhibited by n-hexane, ethyl acetate (EtOAc), or methanol(MeOH) extracts of the fermentation broths of BS-99, BS-23857, and BS-33608. However, the hyphae of P.eugeniae became swollen and malformed after 12 h and stopped growing after 30 h of treatment with theMeOH extract of the BS-99 fermentation broth. Analysis by polymerase chain reaction (PCR), blood agartest plates, and high performance liquid chromatography (HPLC) indicated that, BS-99 produced the antibiotics, iturin A and surfactin, whereas, BS-23857 and BS-33608 only produced surfactin. These data suggest that, the antifungal activity of the B. subtilis BS-99 isolate against P. eugeniae is activated only
    when both iturin A and surfactin are present and not by surfactin alone. Unveiling the antifungal
    mechanism of B. subtilis, BS-99 could promote its commercial development and application as a
    biofungicide for controlling P. eugeniae infections of the wax apple, which is a highly valued fruit in Taiwan.
    關聯: African Journal of Microbiology Research /Vol. 5(14)
    显示于类别:[生物科技系] 期刊論文

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