Meiho University Institutional Repository:Item 987654321/1440
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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/1440


    Title: 人皮膚纖維母細胞之腎上腺素性接受器亞型表現
    Authors: 廖明輝;劉世賢;賴怡君;施以潔;陳芝融;黃漢翔
    Contributors: 國立屏東科技大學獸醫系;國立屏東科技大學獸醫系;國立屏東科技大學獸醫系;美和科技大學生物科技系;美和科技大學美容系
    Date: 2011
    Issue Date: 2011-10-28T01:50:07Z (UTC)
    Abstract: 人的造骨細胞與在體內與纖維母細胞同源,兩種細胞的形態與生理功能非常接近。我們先前證實了穩定表現於人的初代造骨細胞與類造骨細胞MG63之腎上腺素性接受器的訊息RNA(mRNA)或接受器蛋白是為1B與2兩種亞型。已有具權威性的科學研究證實,在纖維母細胞內與所表現的眾多基因,於造骨細胞內亦會表現;而1-腎上腺素性接受器三種亞型的表現,會依據動物種別或所在組織的不同而有差異性;故本實驗的目的,是確認腎上腺素性接受器在人皮膚纖維母細胞的表現與其亞型類別。經以反轉錄聚合酶連鎖反應分析後,在人皮膚纖維母細胞穩定表現的腎上腺素性接受器亞型的mRNA為1A與2兩種。透過免疫螢光法與共軛焦顯微鏡技術,在人皮膚纖維母細胞亦偵測到1A-腎上腺素性接受器之特異性螢光,而陰性對照組特異性螢光極為微弱。本實驗結果顯示,1A-腎上腺素性接受器確實表現於人皮膚纖維母細胞中;未來將對該接受器於皮膚纖維母細胞進行功能性的分析。
    Appears in Collections:[Seminar] International Conference on Health Care and Health Industry

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