Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells

MUIR > Graduate Institute of Health Care > Department of Beauty Science > Research Projects > Item 987654321/1691

Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/1691

Title:	Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells
Authors:	Su, Tzu-Rong Lin, Jen-Jie Chiu, Chien-Chih Chen, Jeff Yi-Fu Su, Jui-Hsin Cheng, Zhi-Jiao Hwang, Wen-Ing Huang, Han Hsiang Wu, Yu-Jen
Contributors:	健康暨護理學院
Keywords:	Melanoma cells;Proteomic analysis;Sinularin
Date:	2011
Issue Date:	2012-09-03T01:15:02Z (UTC)
Abstract:	The extracts from soft corals have been increasingly investigated for biomedical and therapeutic purposes. The aim of this study is to examine and analyze the anti-tumor effects of the genus Sinularia extract sinularin on A2058 melanoma cells using MTT assay, cell migration assay, wound healing assay, flow cytometric analysis, and proteomic analysis. Sinularin dose-dependently (1–5 g/mL) inhibited melanoma cell proliferation while the treatment at identical concentrations suppressed cell migration. Sinularin dose-dependently enhanced apoptotic melanoma cells and caused tumor cell accumulation at G2/M phase, indicating that sinularin exerts apoptosis-induced and cell cycle-delayed activities in A2058 melanoma cells.Comparative proteomic analysiswas conducted to investigate the effects of sinularin at the molecular level by comparison between the protein profiling ofmelanoma cells treated with sinularin and without the treatment. Thirty-five differential proteins (13 upregulated and 22 downregulated) concerning the treatment were identified by liquid chromatography-tandem mass spectrometry. Proteomic data and Western blot displayed the levels of several tumor inhibitory or apoptosis-associated proteins including annexin A1, voltage-dependent anion-selective channel protein 1 and prohibitin (upregulated), heat shock protein 60, heat shock protein beta-1, and peroxiredoxin-2 (downregulated) in A2058 melanoma cells exposed to sinularin. Increased expression of p53, cleaved-caspase- 3, cleaved-caspase-8, cleaved-caspase-9, p21, and Bax and decreased expression of Bcl-2 in sinularin-treated melanoma cells suggest that the anti-tumor activities of sinularin against melanoma cells are particularly correlated with these pro-apoptotic factors. These data provide important information for the mechanisms of anti-tumor effects of sinularin on melanoma cells and may be helpful for drug development and progression monitoring of human melanoma.
Appears in Collections:	[Department of Beauty Science] Research Projects

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