Meiho University Institutional Repository:Item 987654321/1692
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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/1692


    Title: An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells
    Authors: Su, Ching-Chyuan;Su, Jui-Hsin;Lin, Jen-Jie;Chen, Cheng-Chi;Hwang, Wen-Ing;Huang, Han Hsiang;Wu, Yu-Jen
    Contributors: 健康暨護理學院
    Keywords: 13-acetoxysarcocrassolide;Sarcophyton crassocaule;BFTC cells;proteomic analysis
    Date: 2011
    Issue Date: 2012-09-03T01:21:23Z (UTC)
    Abstract: Active compounds from natural products have been widely studied. The
    anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral
    Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT
    assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional
    cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through
    apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the
    ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound
    healing assay. To determine which proteins were affected in the BFTC cells upon treatment,
    a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified
    that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteinswere confirmed by western blotting analysis. This study reveals clues to the potential
    mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it
    suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results
    of this study are also helpful for the diagnosis, progression monitoring and therapeutic
    strategies of transitional cell tumors.
    Appears in Collections:[Department of Beauty Science] Research Projects

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