A quantitative real-time polymerase chain reaction (qPCR) is a robust means by which to monitor
toxin-producing cyanobacteria. However, qPCR usually requires DNA extraction, which is a timeconsuming,
labor-intensive pretreatment. To be able to quickly determine the potential of cyanotoxin
contamination in the field, a rapid pretreatment method for DNA extraction and a portable qPCR
device are needed. In this study, we applied a microwave-based method for the qPCR pretreatment
and a multicolor portable qPCR with UPL and TaqMan probes to quantify toxigenic and total
Microcystis. The method was tested using laboratory cultures of toxigenic Microcystis aeruginosa
PCC7820. The qPCR results showed the cycle thresholds value (Ct value) correlated well with cell
numbers, with detection limit at about 1,000 cells/ml. This scheme was applied in 22 environmental
samples from six drinking water reservoirs (DWRs) in Taiwan. Although the results for qPCR were
about four times higher than those of microscopic observation, good correlation between qPCR and
microscope methods were obtained (r-square: 0.79, P<0.01). The ratios of toxigenic Microcystis to
total Microcystis in two reservoirs, Sin-Shan Reservoir and Shih-men Reservoir, were less than 10%.
In three other reservoirs, Ren-Yi-Tan Reservoir, Nan-Hua Reservoir and Bao-Shan Reservoir, much
higher (>46.1%) ratios were obtained. The scheme may assist quick assessment of the risk
associated with toxic cyanobacteria in DWRs.
