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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/1930


    Title: The antifungal mechanism of Bacillus subtilis against Pestalotiopsis eugeniae and its development for commercial applications against wax apple infection
    Authors: Hsiu Fen Lin;Tsang Hai Chen;Shan Da Liu
    Contributors: 健康暨護理學院
    Keywords: Bacillus subtilis;iturin A;solvent extract;surfactin.
    Date: 2011-07
    Issue Date: 2012-11-06T08:19:58Z (UTC)
    Abstract: The inhibition zones of three Bacillus subtilis isolates, BS-99, BS-23857 and BS-33608 against
    Pestalotiopsis eugeniae were, 13.5, 0, and 0 mm, respectively. BS-99 showed the strongest inhibitory
    activity, whereas, no inhibitory activity was reported for BS-23857 or BS-33608. The spore germination
    rates of P. eugeniae were not significantly inhibited by n-hexane, ethyl acetate (EtOAc), or methanol
    (MeOH) extracts of the fermentation broths of BS-99, BS-23857, and BS-33608. However, the hyphae of P.
    eugeniae became swollen and malformed after 12 h and stopped growing after 30 h of treatment with the
    MeOH extract of the BS-99 fermentation broth. Analysis by polymerase chain reaction (PCR), blood agar
    test plates, and high performance liquid chromatography (HPLC) indicated that, BS-99 produced the
    antibiotics, iturin A and surfactin, whereas, BS-23857 and BS-33608 only produced surfactin. These data
    suggest that, the antifungal activity of the B. subtilis BS-99 isolate against P. eugeniae is activated only
    when both iturin A and surfactin are present and not by surfactin alone. Unveiling the antifungal
    mechanism of B. subtilis, BS-99 could promote its commercial development and application as a
    biofungicide for controlling P. eugeniae infections of the wax apple, which is a highly valued fruit in
    Taiwan.
    Appears in Collections:[Graduate Institute of Applied Health and Biotechnology] Papers

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