Gallic acid (GA) has long been associated with a wide range of biological
activities. In this study, its anti-tumor effect against B16F10 melanoma cells was
examined by MTT assay, cell migration assay, wound healing assay and flow
cytometric analysis. GA with a concentration greater than 400 M shows apoptotic
activity towards B16F10 cells. According to Western blotting, over-expressions of
cleaved forms of caspase-9, caspase-3, and PARP-1, and apropoptic Bax and Bad,
accompanied with under-expressed antiapoptotic Bcl-2 and Bcl-XL indicates that GA
induces B16F10 cell apoptosis via mitochondrial pathway.
