Meiho University Institutional Repository:Item 987654321/2448
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    题名: Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa
    作者: Chin-Shang Ho;Huei-Chuan Shih;Ho-Yih Liu;Shau-Ting Chiu;Mei-Hui Chen;Li-Ping Ju;Ya-Zhu Ko;Yu-Shen Shih;Chaur-Tzuhn Chen;Tsai-Wen Hsu;Yu-Chung Chiang
    关键词: Keteleeria;Conservation;Microsatellites;Simple sequence repeat markers;Pinaceae
    日期: 2014
    上传时间: 2014-07-10
    摘要: Background: Keteleeria davidiana var. formosana (Pinaceae), Taiwan cow-tail fir, is an endangered species listed onthe IUCN Red List of Threatened Species and only two populations remain, both on the Taiwan Island. Sixteenpolymorphic microsatellite loci were developed in an endangered and endemic gymnosperm species, Keteleeriadavidiana var. formosana, and were tested in an additional 6 taxa, K. davidiana var. calcarea, K. davidiana var.chienpeii, K. evelyniana, K. fortunei, K. fortunei var. cyclolepis, and K. pubescens, to evaluate the genetic variationavailable for conservation management and to reconstruct the phylogeographic patterns of this ancient lineage.
    Findings: Polymorphic primer sets were developed from K. davidiana var. formosana using the modified AFLP andmagnetic bead enrichment method. The number of alleles ranged from 3 to 16, with the observed heterozygosityranging from 0.28 to 1.00. All of the loci were found to be interspecifically amplifiable.
    Conclusions: These polymorphic and transferable loci will be potentially useful for future studies that will focus onidentifying distinct evolutionary units within species and establishing the phylogeographic patterns and theprocess of speciation among closely related species.
    關聯: Ho et al. BMC Research Notes 2014, 7:255
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