Meiho University Institutional Repository:Item 987654321/2769
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    题名: Evaluation of chloroplast DNA markers for distinguishing Phalaenopsis species
    作者: Lin, Jhong-Yi;Lin, Bo-Yen;Chang, Ching-Dong;Liao, Sin-Chung;Liu, Yu-Chang;Wu, Wen-Luan;Chang, Ching-Chun
    日期: 2015-07-09
    上传时间: 2015-07-09T06:47:37Z (UTC)
    摘要: Comparative analysis of chloroplast DNA (cpDNA) between two endemic moth orchids in Taiwan, Pha-laenopsis aphrodite subsp. formosana and P. equestris, previously revealed many evolutionary hotspots. Inthis study, we explored 27 pre-screened cpDNA regions to evaluate molecular markers for distinguishingPhalaenopsis species. Fifteen cpDNA markers were highly variable among moth orchids, with polymor-phic information contents ≥8.0. The rps16-trnQ marker showed the best discrimatory power which 15endemic moth orchids could be successfully separated into 13 groups. From the sequences of the sevenselected cpDNA regions, the rps16-trnQ intergenic spacer was the best DNA barcode which 19 endemicmoth orchids could be completely distinguished. The petN-psbM intergenic spacer could be better usedas a DNA barcode to differentiate P. aphrodite subsp. formosana and P. amabilis. Additionally, the inher-itance mode of cpDNA in moth orchids was maternally predominated. Phylogenetic analysis revealednon-monophyletic relationships below the Phalaenopsis genus level. In summary, we have revealed a setof cpDNA markers that could be used for identification and phylogenetic study of Phalaenopsis orchids.© 2015 Elsevier B.V. All rights reserved.
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