Meiho University Institutional Repository:Item 987654321/2934
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    MUIR > Seminar >   >  Item 987654321/2934


    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/2934


    Title: 牛蛙輸卵管雌激素活性的萃取
    Authors: 林依婷;張家禎;林峰源;蔡豐仁
    Keywords: 牛蛙;雌激素
    Date: 2015
    Issue Date: 2015-11-22T04:46:36Z (UTC)
    Abstract: 蛙類輸卵管中含有芳香酶,該酵素是將雄性激素轉化為雌激素的關鍵酵素, 而處於產卵季節的成熟雌蛙其輸卵管內也發現雄性激素有大量轉化為雌激素的 現象。本研究利用養殖的成熟牛蛙冷凍乾燥的輸卵管樣本透過有機溶劑的分劃過 程進行雌激素活性的萃取,以評估是否可應用於化妝保養品中。我們使用牛蛙輸 卵管冷凍乾燥樣本,先經過乙酸乙酯分劃初萃取,再經不同極性的溶劑(正己烷 及甲醇)的分劃萃取,並藉由MCF-7 細胞株MTT 試驗評估雌激素活性,結果顯 示雌激素的活性絕大部分會存留於甲醇層中。然而,甲醇層萃取物的使用濃度越 高卻沒有顯示細胞增殖曲線越高的正相關趨勢,這暗示這甲醇層萃取物可能具有 拮抗雌激素物質存在而影響細胞的增殖。
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