| 摘要: | Objectives This study aimed to identify the prevalence of pathogenic organisms existing on samples collected from diaphragms of stethoscope, and cuffs of blood pressure and to compare before and after decontaminated with disinfectant among the cultured samples in clinical wards. Method A prospective observation study design was used. A total of 104 medical equipment (53 cuffs of blood pressure and 51 stethoscopes) were sampled that included before and after disinfection with Meliseptol rapid (50% kl/kl 1-Propanol 0.07%kl/kl Didecyl Dimethyl Amonium Chloride). Thus, cultures and the pathogenic micro-organisms were identified at Pasteur institute in HCM city. Results For both stethoscopes and blood pressure cuffs, there was a significant difference between the prevalence of pathogenic micro-organisms presents (P <.0001), the number of colonies of pathogenic and a-pathogenic micro-organisms (χ2 =679 and degrees of freedom = 1, P <.0001). The number of Coagulase negative Staphylococus (CoNS) and a-pathogenic micro-organisms (χ2 = 5.79, degrees of freedom = 1, P =.016) (table 4.8.1) before and after being decontaminated with disinfectant. In this study, the results were showed that for Coagualase negative Staphylococcus, the resistant proportions of Cefoxitin 30 and Erythromycin (19/26) were the highest to 73,08 % (19/26), continually, the one of the Azithromycin (24μg) was 69,23 % (18/26), and for three antibiotics Vancomycin, Neltimicin and Teicoplanin 20, their resistant proportions were 0 %. Conclusion This study showed the benefits of disinfectant in prevention and reduction of the contamination through medical equipment in clinical practice. Furthermore, disinfectant can reduce the number of colonies of pathogenic and a-pathogenic micro-organisms, the prevalence of pathogenic micro-organisms presenting on stethoscopes and cuffs of blood pressure meters after disinfection. There are 16 CoNS samples resisted Methicillin (MRCoNS) and 15 MRCoNS had MecA gene identified with Protein Chain Reaction (PCR). MRCoNS presenting on these medical equipments were the evidence of cross contamination or cross-infection from health care workers or patients to blood pressure cuffs and stethoscopes or conversely. The results serve as a reference for health professionals to be aware cross contamination and prevention strategies during patient care. |
