| 摘要: | Pre-germinated brown rice (PGBR) has been found to ameliorate diabetes mellitus, but how it does this is not known. This study investigated the effect and mechanism ofPGBR preventedhyperglycemia in C57BL/6J mice fed a high-fat diet (HFD). Normal six-week-old C57BL/6J mice were randomly divided into three groups. Group 1 was fed standard regular diet (SRD) and group 2 was fed HFD for 16 weeks. In group 3, the mice were fed a HFD with its carbohydrate replaced with PGBR for 16 weeks. Comparing the SRD and HFD groups, we found the HFD group to have higher blood pressure and higher concentrations of blood glucose and HbA1c. The HFD groups had less protein expression of insulin receptor (IR),insulin receptor substrate-1(IRS-1), phosphatidylinositol-3-kinase (PI3K), glucose transporter-4 (GLUT-4) and glucokinase (GCK) and greater expression of glucogen synthase kinase (GSK) in skeletal muscle. The HFD group also had less expression of IR, serine/threonine kinase PI3K-linked protein kinase B (Akt/PKB), AMP-activated protein kinase(AMPK), GCK and peroxisome proliferator-activated receptor PPARin liver. However, in the HFD+PGBR group, the PGBR could reverse the disorders of blood pressure, blood glucose and increased the insulin concentration. The PGBR also increased the IR,IRS-1, PI3K, Akt, GLUT-1 and GLUT-4 proteins, and ameliorated the protein expressions of AMPK, GCK, GSK and PPAR. Together, these findings suggest that PGBR can prevent HFD-induced hyperglycemia through improving insulin levels, insulin receptor, and glucose transporters and enhancing glucose metabolism. |
