| 摘要: | Aims: Pre-germinated brown rice (PGBR) has been proved to ameliorate hyperlipidemia, but the action mechanism is not clear. We focus the biochemical mechanisms of PGBR prevented hyperlipidemia in C57BL/6J mice.
Main methods: Six-week-old mice were randomly divided into three groups. Group 1 was fed standard regular diet, group 2 was fed high-fat diet (HFD) and group 3 was fed HFD with PGBR for 16 weeks.
Key findings: The HFD group has higher concentrations of TG, TC, HDL and non-HDL in the blood. The AI was also enhanced. The TG levels in the liver, and TG, bile acid levels in the feces were enhanced; and the adipocytokines level in adipose tissue was reduced by HFD. The HFD group had higher protein expressions of SREBP-1, SCD-1, FAS, LDLR, and CYP71 in the liver. Moreover, the greater protein expressions of SREBP-1, SCD-1, FAS and the less expression of PPAR-and adiponectin were found in adipose tissue of HFD group. On the other hand, in the HFD+PGBR group, the PGBR could regulate the levels of TG, TC, HDL, non-HDL, AI and adipocytokines. PGBR increased more cholesterol and bile acid exhaust in feces. PGBR inhibited the SREBP-1, SCD-1, FAS and HMGCR proteins, and increased the protein expressions ofLDLR, CYP71 and PPAR- in the liver. The SREBP-1, SCD-1, FASPPAR-and adiponectin proteins of adipose tissue were reversed by PGBR.
Significance: PGBR prevented HFD-induced hyperlipidemia through improving lipid synthesis and metabolism. These findings suggest that PGBR is a recommendable food for controlling hyperlipidemia. |
