| 摘要: | This study investigates the effects of pre-germinated brown rice (PGBR) to treat high fat diet (HFD)-induced metabolic syndrome in C57BL/6J mice. Normal six-week-old mice were randomly divided into three groups. Group 1 was fed standard regular diet for 20 weeks (SRD group). Other mice fed HFD for 16 weeks, confirmed metabolic syndrome, and then fed HFD for 4 weeks (Group 2: HFD group) or changed the HFD that its carbohydrate was replaced with PGBR for 4 weeks (Group 3: HFD+PGBR group). Compared to the SRD group, the HFD group had gained more weight, had higher blood pressure and heart rate as well as higher concentrations of blood glucose and lipids. The HFD group had higher levels of TG in the liver, higher levels of TG and bile acid in the feces, and lower levels of adipocytokine in adipose tissue. The HFD group also had lower protein expressions of IR, IRS-1, IRS-2, PI3K, Akt/PKB, GLUT-1, GLUT-4, GCK and PPAR-in skeletal muscle. The HFD group had higher expressions of SREBP-1, SCD-1, FAS, HMGCR, LDLR, CYP71 and PPAR- in the liver. Furthermore, the HFD group had higher expressions of SREBP-1, SCD-1, FAS, and lower expressions of PPAR-and adiponectin in adipose tissue. In the HFD+PGBR group, PGBR was found to reverse the disorders of blood pressure, heart rate, blood glucose, lipids, and increased insulin concentration. PGBR caused more cholesterol and bile acid to be excreted in feces and increased adipocytokine level in adipose tissue. PGBR increased IR, IRS-1, IRS-2, PI3K, Akt, GLUT-1 and GLUT-4 proteins and ameliorated GCK and PPAR-proteins. PGBR inhibited SREBP-1, SCD-1, FAS and HMGCR proteins, and increased protein expressions ofLDLR, CYP71, PPAR-and adiponectin. Based on these results, we conclude that PGBR can successfully treat HFD-induced metabolic syndrome by improving glucose and lipid synthesis and metabolism. We suggest that patients who want better control of metabolic syndrome considered using PGBR as a staple food. |
