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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3083


    Title: Characterization of Genomic Inheritance of Intergeneric Hybrids between Ascocenda and Phalaenopsis Cultivars by GISH, PCR-RFLP and RFLP
    Authors: Wen-Lin Liu;Huei-Chuan Shih;I-Szu Weng;Ya-Zhu Ko;Chi-Chu Tsai;Chang-Hung Chou;Yu-Chung Chiang
    Date: 2016-04
    Issue Date: 2016-06-18
    Abstract: Background
    The intergeneric hybrids between Ascocenda John De Biase ‘Blue’ and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid progenies, genomic in situ hybridization (GISH) and restriction fragment length polymorphism (RFLP) analysis were conducted to confirm the intergeneric hybridization status.

    Methods/Results
    GISH analysis showed the presence of both maternal and paternal chromosomes in the cells of the putative hybrids indicating that the putative hybrid seedlings were intergeneric hybrids of the two parents. Furthermore, twenty-seven putative hybrids were randomly selected for DNA analysis, and the external transcribed spacer (ETS) regions of nrDNA were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and RFLP analyses to identify the putative hybrids. RFLP analysis showed that the examined seedlings were intergeneric hybrids of the two parents. However, PCRRFLP analysis showed bias to maternal genotype.

    Conclusions
    Both GISH and RFLP analyses are effective detection technology to identify the intergeneric hybridization status of putative hybrids. Furthermore, the use of PCR-RFLP analysis to identify the inheritance of putative hybrids should be carefully evaluated.
    Relation: journal.pone.0153512 April 7, 2016
    Appears in Collections:[Department of Nursing] Papers

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