The ecotourism has quickly developed as the fastest growing industry in recent years. Ecotourism is one kind of tourism industry that also consists of complex service providers like travel agents, hotels, transportations (such as airplane, bus, and ship) restaurants, and destinations, connecting with operational diversity, therefore, those of supported services are linked with a supply chain together, and its management is called ecotourism service supply chain management (ESSCM). Moreover, a low-carbon operation management has also become an important challenge to the ecotourism nowadays. Based on the problems of service and low-carbon operation management made together above, therefore, exploring an effective solution into ecotourism is worthy. Using ESSCM as a strategy for solving industrial problems of service and low-carbon operations management in ecotourism is explored in this paper. The contributions here include clearly explanation of what ESSCM is, and how ESSCM is used with low-carbon operations as a sustainability strategy, and finally a practical case study also examined in the end.