Meiho University Institutional Repository:Item 987654321/3219
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    题名: Suppression of influenza virus infection by the orf virus isolated in Taiwan
    作者: LIN, Fong-Yuan
    关键词: cell culture;immunomodulating;influenza virus;orf virus;plaque assay
    日期: 2016-09-08
    上传时间: 2016-10-19T08:10:12Z (UTC)
    摘要: Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome of double-stranded DNA. ORFV causes contagiouspustular dermatitis or contagious ecthyma in sheep and goats worldwide. In general, detection of viral DNA and observing ORFVvirion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the ORFV in cellculture using irus-containing tissue as inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in central Taiwan
    was successfully grown in cell culture. We further examined the biochemical characteristic of our isolate, including viral genotyping, viralmRNA and protein expression. By electron microscopy, one unique form of viral particle from ORFV infected cellular lysate was demonstratedin the negative-stained field. Moreover, immunomodulating and anti-influenza virus properties of this ORFV were investigated.ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment of ORFV-infected
    cell medium prevents A549 cells from subsequent type A influenza virus (IAV) infection. Similarly, mice infected with ORFV via bothintramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV. In summary, the
    results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further
    proved that pre-exposure of ORFV protects animals from subsequent IAV infection.
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