Functional Analysis of the Short Isoform of Orf Virus Protein OV20.0

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题名:	Functional Analysis of the Short Isoform of Orf Virus Protein OV20.0
作者:	Tseng, Yeu-Yang;Lin, Fong-Yuan;Cheng, Sun-Fang;Tscharke, David;Chulakasian, Songkhla;Chou, Chia-Chi;Liu, Ya-Fen;Chang, Wei-Shan;Wong, Min-Liang;Hsu, Wei-Li
关键词:	Orf virus;OV20.0;PKR
日期:	2016-12-01
上传时间:	2016-12-06T03:02:58Z (UTC)
摘要:	Orf virus (ORFV) OV20.0L is an ortholog of vaccinia virus (VACV) gene E3L. The function of VACV E3 protein as a virulencefactor is well studied, but OV20.0 has received less attention. Here we show that like VACV E3L, OV20.0L encodes two proteins, afull-length protein and a shorter form (sh20). The shorter sh20 is an N-terminally truncated OV20.0 isoform generated when adownstream AUG codon is used for initiating translation. These isoforms differed in cellular localization, with full-length OV20.0 and sh20 found throughout the cell and predominantly in the cytoplasm, respectively. onetheless, both OV20.0 isoformswere able to bind double-stranded RNA (dsRNA)-activated protein kinase (PKR) and dsRNA. Moreover, both isoformsstrongly inhibited PKR activation as shown by decreased phosphorylation of the translation initiation factor eIF2_ subunit andprotection of Sindbis virus infection against the activity of interferon (IFN). In spite of this apparent conservation of function invitro, a recombinant ORFV that was able to express only the sh20 isoform was attenuated in a mouse model.
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