Meiho University Institutional Repository:Item 987654321/3558
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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/3558


    Title: 特用作物雅囊葉萃取、生物活性及其產品化開發
    Authors: 廖信昌;李順美
    Keywords: 雅囊葉;生物活性;萃取分離;產品
    Date: 2018-08-10
    Issue Date: 2018-08-10T01:14:52Z (UTC)
    Abstract: 雅囊葉葉片烘乾後磨碎秤重,並以一定比例之乙醇進行萃取三日,將其萃取物利用抽氣幫浦過濾並利用減壓濃縮機濃縮後,計算獲得其萃取回收率為10.34%。進行雅囊葉萃取物之生物活性分析,分析結果總多醣、總多酚、總三萜類分別為1.42%、1.0%、1.59%。雅囊葉萃取物在DPPH抗氧化能力試驗中,發現隨萃取物濃度增加,抗氧化能力亦增加,在0.125mg/ml,DPPH為24.4%,0.25mg/ml,50.6%﹔0.5mg/ml,85.86%,較對照組,Vit C 10 ppm,31.02% ﹔Vit C 25ppm,69.53%為高,具有優秀之抗氧化能力表現,另外進行了雅囊葉萃取物對正常肝細胞(CL48)的影響試驗,在雅囊葉萃取物濃度125µg/mL處理之CL48細胞與正常肝細胞並無明顯差異,透過MTT細胞毒殺試驗結果顯示,雅囊葉萃取物並不會傷害正常人類肝細胞,以RAW264.7巨噬細胞進行了雅囊葉萃取物在抗發炎能力上的試驗,結果顯示雅囊葉萃取物具有抑制透過酯多醣體LPS誘導巨噬細胞發炎產生發炎物質NO的良好抑制能力。
    Appears in Collections:[Department of Biological Science and Technology] Research Projects

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