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    請使用永久網址來引用或連結此文件: http://ir.meiho.edu.tw/ir/handle/987654321/3886


    題名: (+)-Bornyl p-Coumarate Extracted from Stem of Piper betle Induced Apoptosis and Autophagy in Melanoma Cells
    作者: Wu, Yu-Jen
    Su, Tzu-Rong
    Chang, Chi-I
    Chen, Chiy-Rong
    Hung, Kuo-Feng
    Liu, Cheng
    貢獻者: 健康暨護理學院
    關鍵詞: melanoma
    (+)-bornyl p-coumarate
    apoptosis
    autophagy
    日期: 2020
    上傳時間: 2020-09-24T06:05:13Z (UTC)
    摘要: (+)-Bornyl p-coumarate is an active substance that is abundant in the Piper betle stem
    and has been shown to possess bioactivity against bacteria and a strong antioxidative e ect.
    In the current study, we examined the actions of (+)-bornyl p-coumarate against A2058 and
    A375 melanoma cells. The inhibition e ects of (+)-bornyl p-coumarate on these cell lines were
    assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and the
    underlying mechanisms were identified by immunostaining, flow cytometry and western blotting
    of proteins associated with apoptosis and autophagy. Our results demonstrated that (+)-bornyl
    p-coumarate inhibited melanoma cell proliferation and caused loss of mitochondrial membrane
    potential, demonstrating treatment induced apoptosis. In addition, western blotting revealed that
    the process is mediated by caspase-dependent pathways, release of cytochrome C, activation of
    pro-apoptotic proteins (Bax, Bad and caspase-3/-9) and suppression of anti-apoptotic proteins
    (Bcl-2, Bcl-xl and Mcl-1). Also, the upregulated expressions of p-PERK, p-eIF2 , ATF4 and
    CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP) after treatment indicated that
    (+)-bornyl p-coumarate caused apoptosis via endoplasmic reticulum (ER) stress. Moreover, increased
    expressions of beclin-1, Atg3, Atg5, p62, LC3-I and LC3-II proteins and suppression by autophagic
    inhibitor 3-methyladenine (3-MA), indicated that (+)-bornyl p-coumarate triggered autophagy in the
    melanoma cells. In conclusion, our findings demonstrated that (+)-bornyl p-coumarate suppressed
    human melanoma cell growth and should be further investigated with regards to its potential use as
    a chemotherapy drug for the treatment of human melanoma.
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