資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://ir.meiho.edu.tw/ir/handle/987654321/3914
|
| 題名: | The genetic relationships of Indian jujube (Ziziphus mauritiana Lam.)cultivars using SSR markers |
| 作者: | Chiou, Chu-Ying
Shih, Huei-Chuan
Tsai, Chi-Chu
Jin, Xiao-Lei
Ko, Ya-Zhu
Mantiquilla, Junaldo A.
Weng, I-Szu
Chiang, Yu-Chung |
| 貢獻者: | 健康暨護理學院 |
| 關鍵詞: | Genetic diversity
Genetic structure
Microsatellite DNA |
| 日期: | 2020-09 |
| 上傳時間: | 2020-11-05T08:41:03Z (UTC)
|
| 摘要: | The genetic relationships among 24 Indian jujube cultivars (Ziziphus mauritiana Lam.) were evaluated by genotyping the microsatellite loci using simple sequence repeat (SSR) markers. The SSR loci were scored by fluorescent labelling and automated detection systems for the high-throughput capillary electrophoresis and high-resolution gel electrophoresis. Out of the 29 newly characterized SSR loci, 26 were considered as polymorphic with a total of 181 alleles obtained. The number of alleles ranged from 2–12, while the polymorphism information content ranged from 0.08–0.83, and the expected and observed heterozygosity were 0.04–0.83 and 0.04–0.82, respectively. The allele pattern of Indian jujube for all SSR loci confirmed its karyotype as tetraploid. Similarity coefficients and UPGMA dendrogram revealed that the Taiwanese cultivars consisted of a large ‘A’ clade, which is further divided into ‘A1’ and ‘A2’ groups, and the ‘B’ clade where both are rooted by the wild accession, ‘Chad native’. These four genetic clusters were supported by the results of PCoA and the assignment test. The excess of heterozygotes based on F-statistics was attributed to its mating system as outcrossing and self-incompatible, and the introgression of the presumed mutation-derived cultivars with genetic admixture. Based on this study, SSR markers offer valuable information on the genetic relationship of this tropical fruit tree which is basically in agreement with the genealogy of its breeding history. |
| 顯示於類別: | [護理系] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| The genetic relationships of Indian jujube (Ziziphus mauritiana Lam.)cultivars using SSR markers.pdf | | 1015Kb | Adobe PDF | 0 | 檢視/開啟 |
|
在MUIR中所有的資料項目都受到原著作權保護.
|
