Chlorogenic acid (CGA) is a polyphenol compound found in a variety of foods, including coffee, tea, cherries, and apples. It has been found by a number of studies to affect viability of human cancer cells. No study has investigated its effect on esophageal squamous cell carcinoma (ESCC) metastasis or the molecular mechanism underlying its effect on this disease. To find out, we first used a Taiwanese ESCC cell line CE81T/VGH to create CE81T-M4 cells. We treated these cells, which were found motile after four rounds of repeated selection by transwell chamber, with chlorogenic acid for 24 hours and then assessed metastasis. Scratch migration and transwell assays found that chlorogenic acid had inhibited the ability of the cells to migrate and invade.
