A KDEL Retrieval System for ER-Golgi Transport of

Meiho University Institutional Repository > 健康暨護理學院 > 美容系 > 期刊論文 > Item 987654321/3389

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.meiho.edu.tw/ir/handle/987654321/3389

题名:	A KDEL Retrieval System for ER-Golgi Transport of
作者:	Wu, Yu-Jen;Wang, Robert YL;Chen, Han-Shan;Chen, Chih-Jung
关键词:	Japanese encephalitis virus;GRP78;KDEL receptor;ER-Golgi retrieval;viral particles
日期:	2017-10-30
上传时间:	2017-10-30T06:54:53Z (UTC)
摘要:	Evidence has emerged that RNA viruses utilize the host secretory pathway for processing and trafficking mature viral particles and for exiting the infected cells. Upon completing the complex assembly process, the viral particles take advantage of the cellular secretory trafficking machinery for their intracellular trafficking toward the Golgi organelle and budding or export of virions. In this study, we showed that Japanese encephalitis virus (JEV)-induced extracellular GRP78 contains no KDEL motif using an anti-KDEL-specific antibody. Overexpression of the KDEL-truncated GRP78 in the GPR78 knocked down cells significantly reduced JEV infectivity, suggesting that the KDEL motif is required for GRP78 function in the release of JE viral particles. In addition, we demonstrated the KDELR protein, an ER-Golgi retrieval system component, is associated with viral envelope proteins and is engaged in the subcellular localization of viral particles in Golgi. More importantly, accumulation of intracellular virions was observed in the KDELR knocked down cells, indicating that the KDELR protein mediated the intracellular trafficking of JE viral particles. Altogether, we demonstrated that intracellular trafficking of JE assembled viral particles was mediated by the host ER-Golgi retrieval system prior to exit by the secretory pathway.
显示于类别:	[美容系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
Yu-Jen Wu;Robert YL Wang; Han-Shan Chen;Chih-Jung Chenviruses-08-00044.pdf		4408Kb	Adobe PDF	1	检视/开启

检视Licence

在MUIR中所有的数据项都受到原著作权保护.

数据加载中.....