Application of response surface methodology and quantitative methodology and quantitative NMR for the optimum extraction, characterization, and quantitation of Antrodia cinnamomea triterpenoids

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Title:	Application of response surface methodology and quantitative methodology and quantitative NMR for the optimum extraction, characterization, and quantitation of Antrodia cinnamomea triterpenoids
Authors:	吳東穎
Contributors:	健康暨護理學院
Keywords:	Abbreviations AC Antrodia cinnamomea EEAC Ethanolic extract from A. cinnamomea FEA The ethyl acetate fraction from EEAC (the triterpenoid-rich fraction) UAE Ultrasonic-assisted extraction
Date:	2023-12-13
Issue Date:	2023-12-15T05:34:47Z (UTC)
Abstract:	Antrodia cinnamomea (AC) is a treasured Asian medicinal mushroom, which has attracted attention due to recent research on its effectiveness in targeting a variety of serious ailments such as cancer and liver diseases. Among different A. cinnamomea constituents, triterpenoids are regarded as the most therapeutically attractive components because of their anti-inflammatory and cytotoxic activities. In the present study, we proposed a mathematical and statistical extraction protocol to evaluate the concentrations of total ergostane and lanostane triterpenoid derivatives from the ethanolic extract of the wild fruiting bodies of A. cinnamomea (EEAC) by utilizing response surface methodology (RSM) and quantitative NMR (qNMR) approaches. The optimum response surface model showed that the variations of the investigated response variables reached more than 90%, suggesting that the developed model is accurate in explaining response variability. Furthermore, the EEAC major characteristic triterpenoids were quantified through the comparison of the HPLC-tandem MS results with those of the qNMR results. The precision of the used techniques was also evaluated. The experimental design of the EEAC optimum extraction procedure obtained by using RSM and qNMR enabled accurate characterization and quantitation of A. cinnamomea triterpenoids.
Appears in Collections:	[Department of Food Science and Nutrition] Papers

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