Meiho University Institutional Repository:Item 987654321/4216
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    Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/4216


    Title: Comparative chemical profiling of leaf essential oils from Cinnamomum kanehirae and related species using steam distillation and solvent extraction: Implications for plant-based classification
    Authors: Chen, Wen-Hui
    Ko, Ya-Zhu
    Chang, Hsiu-Chun
    Chang, Chui-Shiang
    Hung, Kuo-Hsiang
    Shih, Huie-Chuan
    Ju, Li-Ping
    Shiao, Meng-Shin
    Chiang, Yu- Chung
    Contributors: 健康暨護理學院
    Keywords: Cinnamomum kanehirae
    Cinnamomum micranthum
    Cinnamomum camphora
    Essential oil
    Gas chromatography/mass spectrometry (GC/ MS)
    Chemotype
    Date: 2024-06-14
    Issue Date: 2024-06-14T08:47:03Z (UTC)
    Abstract: Cinnamomum kanehirae Hayata, belonging to Lauraceae family, is an indigenous and endangered species of considerable economic importance in Taiwan. It plays a crucial role as the host for the economically valuable saprotrophic fungus, Taiwanofungus camphorates. However, accurate species identification poses a challenge due to the similarity in morphological features and frequent natural hybridization with closely related species. Acquiring high-quality and pure leaf oils becomes imperative for precise species identification and producing superior goods. In this study, our objective was to establish methodologies for analyzing the chemical composition of leaf essential oils and subsequently apply this knowledge to differentiate among three Cinnamomum species. Gas chromatography-mass spectrometry (GC/MS) was employed to scrutinize the chemical makeup of leaf essential oils from three closely related species: C. kanehirae, C. micranthum, and C. camphora. We utilized Steam Distillation (SD) and steam distillation-solvent extraction (SDSE) methods, with the SDSE-Hexane approach chosen for optimization, enhancing extraction efficiency and ensuring essential oil purity. Through the SDSE-Hexane method, we identified seventy-four compounds distributed across three major classes: monoterpenes hydrocarbons (0.0–7.0 %), oxygenated monoterpenes (3.8–90.9 %), sesquiterpenes hydrocarbons (0.0–28.3 %), and oxygenated sesquiterpenes (1.6–88.1 %). Our findings indicated the presence of more than one chemotype in both C. kanehirae and C. camphora, whereas no specific chemotype could be discerned in C. micranthum. Furthermore, clustering based on chemotypes allowed for the differentiation of samples from the three species. Notably, we demonstrated that the chemical
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