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http://ir.meiho.edu.tw/ir/handle/987654321/1179
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| 題名: | Autologous bone marrow cell implantation attenuates left ventricular remodeling and improves heart function in porcine myocardial infarction: An echocardiographic, six-month angiographic, and molecular–cellular study |
| 作者: | Steve Leu;Cheuk-Kwan Sun;Jiunn-Jye Sheu;Li-Teh Chang;Chun-Man Yuen;Chia-Hung Yen;Chiang-Hua Chiang;Sheung-Fat Ko;Sung-Nan Pei;Sarah Chua;Ali A. Youssef;Chiung-Jen Wu;Hon-Kan Yip |
| 貢獻者: | Department of Cardiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of General Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of Cardiovascular Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Basic Science, Nursing Department, Meiho Institute of Technology, Pingtung, Taiwan, ROC;Department of Neurosurgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC f Department of Life Science, National Pingtung University of Science and Technology, Taiwan, ROC;Department of Veterinary Medicine, National Pingtung University of Science and Technology, Taiwan, ROC;Department of Radiology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of Hematology;Center for Translational Research in Biomedical Sciences, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC;Department of Cardiology, Suez Canal University Hospital, Ismailia, Egypt |
| 關鍵詞: | Acute myocardial infarct;Autologous bone marrow-derived stem cell therapy;Molecular–cellular mechanisms;Mini-pigs |
| 日期: | 2011 |
| 上傳時間: | 2011-09-27T02:46:11Z (UTC)
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| 摘要: | Background: We investigated the potential benefits and the underlying mechanisms of autologous bone marrow-derived mononuclear cell (BMDMNC) implantation in a porcine model of acute anterior wall myocardial infarction (AAWMI) by studying 6-month left ventricular (LV) function and LV remodeling.
Methods: After being aspirated from the iliac crest and cultured for 1 week, BMDMNCs were implanted immediately after AAWMI induction through the left anterior descending artery ligation. Thirty male minipigs (16–18 kg) were equally divided into group 1 [AAWMI plus saline injection into infarct-ischemia area (IA)], group 2 (AAWMI plus 3.0×107 BMDMNC transplantation into non-IA), group 3 (AAWMI plus 3.0×107 BMDMNC transplantation into IA), group 4 (sham control plus 3.0×107 BMDMNC transplantation into LV myocardium), and group 5 (normal control).
Results: By day 90, echocardiography demonstrated an increased LV end-diastolic and end-systolic dimensions but reduced LV ejection fraction (LVEF) in groups 1 and 2 than in other groups (all pb0.01). Six-month angiographic study showed a lower LVEF and wall motion score but a higher mitral regurgitation in groups 1 and 2 than in other groups (all pb0.01). In IA and peri-infarct area, the number of small vessels and mRNA expressions of endothelial nitric oxide synthase, Bcl-2, interleukin (IL)-10, and peroxisome proliferatoractivated receptor-γ coactivator-1α were lower, whereas the number of apoptotic nuclei, caspase-3, Bax, endothelin-1, IL-8, and matrix metalloproteinase was higher in groups 1 and 2 than in other groups (all pb0.01).
Conclusions: Autologous BMDMNC transplantation into IA rather non-IA improves LV function and reduces LV remodeling via eliciting a broad-spectrum of molecular–cellular defensive mechanisms. |
| 關聯: | International Journal of Cardiology 150 (2011) 156–168 |
| 顯示於類別: | [護理系] 期刊論文
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