Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties

MUIR > Graduate Institute of Health Care > Department of Beauty Science > Papers > Item 987654321/1233

Please use this identifier to cite or link to this item: http://ir.meiho.edu.tw/ir/handle/987654321/1233

Title:	Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties
Authors:	Ming-Hsiu Wu;Tzu-Ming Pan;Yu-Jen Wu;Sue-Joan Chang;Ming-Song Chang;Chun-Yi Hu
Contributors:	Department of Nutrition and Health Science, Fooyin University, Kaohsiung County, Taiwan;Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan;Department of Beauty Science, Meiho Institute of Technology, Pingtung County, Taiwan;Department of Biology and Institute of Biodiversity, National Cheng Kung University, Tainan, Taiwan;Department of Surgery, Fooyin Hospital, Pingtung County, Taiwan;Research and Certificate Center of Health Food, Fooyin University, Kaohsiung County, Taiwan
Keywords:	Probiotics;Exopolysaccharide (EPS);Cytokine;Macrophage;Antimicrobial test
Date:	2010-11
Issue Date:	2011-09-28T04:06:58Z (UTC)
Abstract:	Exopolysaccharides (EPSs) are a metabolite of probiotics which have gained wide interest recently, but little is known about their function. EPS was isolated from Bifidobacterium longum BCRC 14634 and sterilized by 0.22 μm filter. The proliferation of J77A.1 macrophages and their secretion of the anti-inflammatory cytokine interleukin-10 (IL-10) was elevated after treatment with heat-killed B. longum or 5 μg/mL EPS. The endotoxin, lipopolysaccharides (LPS), a potent inducer of pro-inflammatory cytokine tumor necrosis factor-α (TNF-α), significantly suppressed the growth of J77A.1 cells, and induced the secretion of TNF-α from J774A.1 cells. Furthermore, 24h pretreatment with 5 μg/ml EPS suppressed 100 ng/ml LPS-induced cell growth inhibition and release of TNF-α from J774A.1 cells. Additional experiments showed that 80 μg/mL EPS had antimicrobial activity against 7 species of food-spoilage and infection bacteria. Our results suggest that EPS from B. longum might be useful as a mild immune modulator for macrophages, contributing to the capacity of B. longum to fight against gastrointestinal infections, and even some food-spoilage microbe.
Relation:	Int J Food Microbiol. 2010 Nov 15;144(1):104-10. Epub 2010 Sep 15.
Appears in Collections:	[Department of Beauty Science] Papers

Files in This Item:

File	Description	Size	Format
index.html	Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties	0Kb	HTML	2228	View/Open

Loading...