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| 題名: | Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells |
| 作者: | Su, Tzu-Rong
Lin, Jen-Jie
Chiu, Chien-Chih
Chen, Jeff Yi-Fu
Su, Jui-Hsin
Cheng, Zhi-Jiao
Hwang, Wen-Ing
Huang, Han Hsiang
Wu, Yu-Jen |
| 貢獻者: | 健康暨護理學院 |
| 關鍵詞: | Melanoma cells;Proteomic analysis;Sinularin |
| 日期: | 2011 |
| 上傳時間: | 2012-09-03T01:15:02Z (UTC)
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| 摘要: | The extracts from soft corals have been increasingly investigated for biomedical and therapeutic
purposes. The aim of this study is to examine and analyze the anti-tumor effects of
the genus Sinularia extract sinularin on A2058 melanoma cells using MTT assay, cell migration
assay, wound healing assay, flow cytometric analysis, and proteomic analysis. Sinularin
dose-dependently (1–5 g/mL) inhibited melanoma cell proliferation while the treatment
at identical concentrations suppressed cell migration. Sinularin dose-dependently
enhanced apoptotic melanoma cells and caused tumor cell accumulation at G2/M phase,
indicating that sinularin exerts apoptosis-induced and cell cycle-delayed activities in A2058
melanoma cells.Comparative proteomic analysiswas conducted to investigate the effects of
sinularin at the molecular level by comparison between the protein profiling ofmelanoma
cells treated with sinularin and without the treatment. Thirty-five differential proteins (13
upregulated and 22 downregulated) concerning the treatment were identified by liquid
chromatography-tandem mass spectrometry. Proteomic data and Western blot displayed
the levels of several tumor inhibitory or apoptosis-associated proteins including annexin
A1, voltage-dependent anion-selective channel protein 1 and prohibitin (upregulated),
heat shock protein 60, heat shock protein beta-1, and peroxiredoxin-2 (downregulated) in
A2058 melanoma cells exposed to sinularin. Increased expression of p53, cleaved-caspase-
3, cleaved-caspase-8, cleaved-caspase-9, p21, and Bax and decreased expression of Bcl-2 in
sinularin-treated melanoma cells suggest that the anti-tumor activities of sinularin against
melanoma cells are particularly correlated with these pro-apoptotic factors. These data
provide important information for the mechanisms of anti-tumor effects of sinularin on
melanoma cells and may be helpful for drug development and progression monitoring of
human melanoma. |
| 顯示於類別: | [美容系] 研究計畫
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