| 摘要: | Skin fibroblasts modulate tissue repair, wound
healing and immunological responses. Adrenergic receptors
(ARs) mediate important physiological functions, such as
endocrine, metabolic and neuronal activity. In this study, the
expression α1A-ARs in human skin fibroblasts is examined
and verified. Regulatory effects of α1-agonist cirazoline on
cell migration and the production of transforming growth
factor β1 (TGF-β1), insulin-like growth factor 1 (IGF-1),
hyaluronan (HA), fibronectin and procollagen type I
carboxy-terminal peptide (PIP) by human skin fibroblasts
are assessed and validated. α1A-AR mRNA and protein were
found in human skin fibroblasts WS1. Exposure of cirazoline
doubled skin fibroblast migration and the increase in cell
migration was attenuated by α1-antagonist prazosin.
TGF-β1 mRNA and production were enhanced after exposure to cirazoline and IGF-1 production was also increased
after treatment with cirazoline. Exposure to cirazoline
also enhanced HA and PIP production. The increases in
TGF-β1, IGF-1, HA and PIP production were partially
abolished in fibroblasts transfected with α1A-AR short interfering
RNAs, indicating that α1A-ARs are involved in the
cirazoline-induced increases in TGF-β1, IGF-1, HA and PIP
production. Thus, α1A-ARs are stably expressed and stimulate
cell migration and TGF-β1, IGF-1, HA and PIP production
in human skin fibroblasts. Moreover, TGF-β1, IGF-1,
HA and PIP production and the cell migration of human skin
fibroblasts are possibly modulated by natural catecholamines
produced by the endocrine system or sympathetic innervation,
which could directly or indirectly participate in cytokine secretion,
fibroblast migration and matrix production of wound
healing in the skin. |
