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Item 987654321/2769
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請使用永久網址來引用或連結此文件:
http://ir.meiho.edu.tw/ir/handle/987654321/2769
題名:
Evaluation of chloroplast DNA markers for distinguishing Phalaenopsis species
作者:
Lin, Jhong-Yi
;
Lin, Bo-Yen
;
Chang, Ching-Dong
;
Liao, Sin-Chung
;
Liu, Yu-Chang
;
Wu, Wen-Luan
;
Chang, Ching-Chun
日期:
2015-07-09
上傳時間:
2015-07-09T06:47:37Z (UTC)
摘要:
Comparative analysis of chloroplast DNA (cpDNA) between two endemic moth orchids in Taiwan, Pha-laenopsis aphrodite subsp. formosana and P. equestris, previously revealed many evolutionary hotspots. Inthis study, we explored 27 pre-screened cpDNA regions to evaluate molecular markers for distinguishingPhalaenopsis species. Fifteen cpDNA markers were highly variable among moth orchids, with polymor-phic information contents â¥8.0. The rps16-trnQ marker showed the best discrimatory power which 15endemic moth orchids could be successfully separated into 13 groups. From the sequences of the sevenselected cpDNA regions, the rps16-trnQ intergenic spacer was the best DNA barcode which 19 endemicmoth orchids could be completely distinguished. The petN-psbM intergenic spacer could be better usedas a DNA barcode to differentiate P. aphrodite subsp. formosana and P. amabilis. Additionally, the inher-itance mode of cpDNA in moth orchids was maternally predominated. Phylogenetic analysis revealednon-monophyletic relationships below the Phalaenopsis genus level. In summary, we have revealed a setof cpDNA markers that could be used for identification and phylogenetic study of Phalaenopsis orchids.© 2015 Elsevier B.V. All rights reserved.
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