資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://ir.meiho.edu.tw/ir/handle/987654321/3534
|
| 題名: | PGBR extract ameliorates TNF-a induced insulin |
| 作者: | Chen, Fu-Chih;Shen, Kuo-Ping;Chen, Jin-Bor;Lin, Hui-Li;Hao, Chi-Long;Yen, Hsueh-Wei;Shaw, Shyh-Yu |
| 關鍵詞: | Pre-germinated;brown rice;Insulin;TNF-a;HepG2 cell |
| 日期: | 2018-04-18 |
| 上傳時間: | 2018-04-18T05:13:58Z (UTC)
|
| 摘要: | Pre-germinated brown rice (PGBR) could ameliorate metabolic syndrome, however,
not much research estimates the effect of PGBR extract on insulin resistance. The aim of this
study is to examine the effects of PGBR extract in TNF-a induced insulin resistance. HepG2
cells, hepatocytes, were cultured in DMEM medium and added with 5 mM insulin or with insulin
and 30 ng/ml TNF-a or with insulin, TNF-a and PGBR extract (50, 100, 300 mg/ml). The glucose
levels of the medium were decreased by insulin, demonstrating insulin promoted glucose uptake
into cell. However, TNF-a inhibited glucose uptake into cells treated with insulin. Moreover,
insulin increased the protein expressions of AMP-activated protein kinase (AMPK), insulin
receptor substrate-1 (IRS-1), phosphatidylinositol-3-kinase-a (PI3K-a), serine/threonine kinase
PI3K-linked protein kinase B (Akt/PKB), glucose transporter-2 (GLUT-2), glucokinase (GCK),
peroxisome proliferator activated receptor-a (PPAR-a) and PPAR-g. TNF-a activated p65 and
MAPKs (JNK1/2 and ERK1/2) which worsened the expressions of AMPK, IRS-1, PI3K-a, Akt/
PKB, GLUT-2, GCK, glycogen synthase kinase-3 (GSK-3), PPAR-a and PPAR-g. Once this relationship
was established, we added PGBR extract to cell with insulin and TNF-a. We found glucose
levels of medium were lowered and that the protein expressions of AMPK, IRS-1, PI3K-a, Akt/
PKB, GLUT-2, GCK, GSK-3, PPAR-a, PPAR-g and p65, JNK1/2 were also recovered. In conclusion,
this study found that TNF-a inhibited insulin stimulated glucose uptake and aggravated |
| 顯示於類別: | [護理系] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| 沈國屏2018-KJMS (PGBRE).pdf | | 1170Kb | Adobe PDF | 0 | 檢視/開啟 |
|
在MUIR中所有的資料項目都受到原著作權保護.
|
