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請使用永久網址來引用或連結此文件:
http://ir.meiho.edu.tw/ir/handle/987654321/3729
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| 題名: | Genomic Features, Comparative Genomic Analysis, |
| 作者: | Liang, Chih-Yu;Yang, Chih-Hui;Lai, Chung-Hsu;Huang, Yi-Han;Lin, Jiun-Nong |
| 關鍵詞: | Chryseobacterium arthrosphaerae;genomic features;comparative genomics;antimicrobial |
| 日期: | 2019-09-02 |
| 上傳時間: | 2019-09-02T05:56:27Z (UTC)
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| 摘要: | Abstract: Bacteria belonging to the genus Chryseobacterium are ubiquitously distributed in natural
environments, plants, and animals. Except C. indologenes and C. gleum, other Chryseobacterium
species rarely cause human diseases. This study reported the whole-genome features, comparative
genomic analysis, and antimicrobial susceptibility patterns of C. arthrosphaerae ED882-96 isolated
in Taiwan. Strain ED882-96 was collected from the blood of a patient who had alcoholic liver
cirrhosis and was an intravenous drug abuser. This isolate was initially identified as C. indologenes
by using matrix-assisted laser desorption ionization–time of flight mass spectrometry. The analysis
of 16S ribosomal RNA gene sequence revealed that ED882-96 shared 100% sequence identity with
C. arthrosphaerae type strain CC-VM-7T. The results of whole-genome sequencing of ED882-96
showed two chromosome contigs and one plasmid. The total lengths of the draft genomes of
chromosome and plasmid were 4,249,864 bp and 435,667 bp, respectively. The findings of both in silico
DNA–DNA hybridization and average nucleotide identity analyses clearly demonstrated that strain
ED882-96 was a species of C. arthrosphaerae. A total of 83 potential virulence factor homologs were
predicted in the whole-genome sequencing of strain ED882-96. This isolate was resistant to all tested
antibiotics, including _-lactams, _-lactam/_-lactamase inhibitor combinations, aminoglycosides,
fluoroquinolones, tetracycline, glycylcycline, and trimethoprim-sulfamethoxazole. Only one antibiotic
resistance gene was recognized in the plasmid. By contrast, many antibiotic resistance genes were
identified in the chromosome. The findings of this study suggest that strain ED882-96 is a highly
virulent and multidrug-resistant pathogen. Knowledge regarding genomic characteristics and
antimicrobial susceptibility patterns provides valuable insights into this uncommon species. |
| 顯示於類別: | [生物科技系] 期刊論文
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